Intermediate

Part:BBa_K218025:Design

Designed by: Prima Moinul   Group: iGEM09_Calgary   (2009-10-18)

autoinducer inactivation enzyme (aiiA) and Double terminators


The autoinducer inactivation enzyme (aiiA) followed by double terminators is an intermediate part in the construction of the AHL-degrading response circuit.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 99
  • 1000
    COMPATIBLE WITH RFC[1000]

Part Design

This biological fragment is constructed from two previously existing Biobricked parts. The autoinducer inactivation gene codes for a protein which facilitates the enzymatic degradation of AHL (N-acyl-homoserine lactones)- a Quorum Sensing Molecule. Only the aiiA gene was submitted to the registry in 2004 as(Part:BBa_C016). It is followed by the double terminators (Part:BBa_B0015). This construct is also available with a Ribosome Binding Site leading the construct (Part:BBa_K218026).


Source

The source of the BBa_B0015 and BBa_C0160 was the Registry of Standard Biological Parts.

References

Miller, M. B. & Bassler, B. L. 2001. Quorum sensing in bacteria. Annu Rev Microbiol 55:165-199